QI need a total of 500 mg of the product for animal experiments, but it won't be used up all at once. How should I dissolve it?
ADetermining the amount based on solution clarity.1. If the prepared solution is clear, it can be prepared in a larger amount for one-time use and stored at 4°C. It is recommended to prepare the solution once a week, as prolonged storage may lead to loss of efficacy. 2. If the prepared solution is a suspension, it is recommended to prepare and use immediately each time.
QCan Tween-80 be used instead of Tween-20?
ATween-20 is not recommended. Tween-80 has better tolerance and has been used as a tool to assess the behavioral effects of experimental drugs and toxins without significant side effects.
Reference: Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.
QHow to dilute working solutions?
ACalculate the dilution factor and volume of the stock solution needed based on the concentration of your working solution. It is recommended to use solvents such as water, saline solution, PBS, or culture medium for dilution. For cell experiments, culture medium is suitable.
Slowly add the appropriate solvent to the stock solution until you reach the desired concentration of your working solution. Gentle vortexing or pipetting can help mix the solution evenly.
Most of our compounds are lipophilic, and precipitation may occur when using PBS or culture medium to dilute the working solution. It is normal and the product can usually be completely dissolved by sonication.
It is recommended to prepare and use the working solution immediately after dilution
QCan PEG400 be used instead of PEG300?
APEG300 has moderate viscosity, which facilitates the dissolution and administration of drugs, while also providing good tolerability without adverse effects on animals. PEG400 can replace PEG300.
PEG600 is not very recommended because its melting point is close to room temperature.
QHow to set the concentration of stock solution?
AThe concentration of stock solution should be lower than the solubility provided on the official website. Within this range, the concentration of stock solution should be set based on that of working solution. For cell experiments, it is recommended to set the stock solution concentration at least 1000 times higher than that of working solution.